Course 4: Polymerase Chain Reaction (PCR) Technology (14 hours)

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Science

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Level: Certificate

Duration : 14hours

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Course Content

  • Guidelines for PCR
  • Principle of PCR
  • Critical factors for successful PCR
  • Designing PCR primers
  • Standard practices in a PCR Laboratory
  • Troubleshooting and prevention of carryover contamination
  • Optimization strategies for PCR
  • Detection of PCR products by gel electrophoresis
  • Methods of purification and quantification of PCR products
  • Methods of labelling of PCR products (PCR probes) and visualization of label
  • Cloning of PCR products: TOPO cloning
  • DNA sequence analysis of PCR product 

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